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The general procedure for deamination of peptides with N-Bn function under hydrogenolysis conditions (III). To a solution of peptide ester 33 (1 mmol) in 1,2-dimethoxyethane (3 mL) 10% Pd/C (0.05 g) was added, and the mixture was hydrogenated at room temperature and normal pressure. The reaction was left to proceed until the disappearance of the C=O ester function was completed (around 30-40 h). Then, to the mixture water was added until a white precipitate was fully dissolved and the catalyst was then filtered off. The resulting precipitate was dissolved in acetone and allowed to stand at 5 °C until complete precipitation. The formed solid was filtered, washed with acetone and dried. The solid was purified by RP-HPLC.
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One of the drawbacks to this approach was that we needed to recompile every time we made even a small change to the code. This slowed everything else we were doing down and because we couldn't always have someone waiting for the application to finish building. 827ec27edc