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Well established procedures were used to assess the effects of cocaine on the heart. All procedures were carried out in accordance with the Animals (Scientific Procedures) Act 1986. Cocaine (0, 0.2, 0.5, 1.0 or 2.0 mg/kg, i.p.) was administered to anaesthetised male C57/Bl6 mice, and the heart rate and blood pressure were recorded, respectively, using a mouse tail-cuff model and a catheter technique. In a separate experiment, mice were administered cocaine (2.0 mg/kg, i.p.), and after 4 h they were anaesthetised and blood was sampled through a cardiac puncture, and brain tissue was isolated for analysis of dopamine and serotonin using high performance liquid chromatography with electrochemical detection. Cocaine Heart Blood Brain
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